What is a tool to create a -S-S- bridge?

More Sangita Kachhap's questions See All

To synthesized thiazine,oxazine etc. heterocyclic form chalcone derivatives ?

methods for these heterocyclic compounds.

17 January 2024 4,075 1 View

Which software is used in place of chemdraw?

name of software

08 June 2023 590 3 View

Anyone can suggest best protein peptide docking server/tools/ for binding energy calculation?

I possess 250 peptides and one target protein, prompting my search for tools/servers that allow docking of multiple peptides simultaneously. While I have already utilized HPPdock, I am interested...

15 May 2023 9,783 2 View

How do I generate ROC curves specifically in ArcGIS environment for validation of Groundwater Potential zones?

I have generated groundwater potential zones using AHP technique. Now I want to validate my result with existing yield data (77 wells). How can I know the sensitivity and specificity of my study?

24 February 2019 292 7 View

Is it necessary, for modelling, only similar organism or similar function protein will be take for target prediction ?

I have a template i.e. transcription factors protein my aim is to predict 3D structure of that protein. I am using PSI-BLAST for target search, but in PSI-BLAST, similarity is showing...

06 August 2018 7,807 1 View

Can i take target protein which have E-value worse than threshold for modelling?

In PSI-BLAST result, i found a target sequence which have e-value 1.8. Can i take this protein as my template protein target for homology modelling?

06 August 2018 9,630 2 View

Can i use PSI-BLAST for template selection of Homlogy Modelling instated of BLASTP ?

I have a 265 long aa sequence and my aim is to design a 3D structure of this sequence.In BLASTP, sequence showing 26% query cover and 81% ident. I tried modeller9.20, I-TASSER, Phyre2,...

03 August 2018 7,678 2 View

MRM scan using QTRAP400

Hi experts, Can we use MRM transition using higher m/z value using QTRAP4000 (for example 1300.3>350.2)? Just curious. QTRAP4000 can scan maximum 1200 m/z then how can it scan for >1200 m/z...

26 July 2017 3,305 6 View

Quantitation using mass spectrometry?

Hello, I have a simple question on quantitation using mass spectrometry with an example. Here is an example: Experimental: 50mg sample was used to extract metabolites, the sample was extracted,...

23 January 2017 3,931 8 View

Calibration curve and internal standards?

Hello experts, I want to build a calibration curve by purchasing the lipid mixture containing several group of lipids with different concentration (For example: PC, PE, CE, SM with concentration...

24 October 2016 1,916 5 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Sangita Kachhap

Thanks Alexander, but I am looking for any in silico tool (any server/program) to create it.

Annemarie Honegger

Disulfide by Design http://cptweb.cpt.wayne.edu/DbD/

Disulfide by Design 2 http://cptweb.cpt.wayne.edu/DbD2/ is the more recent version.

also see: http://www.biomedcentral.com/1471-2105/14/346

Thank Ma,am

its working. Actually I have tried it before but due to java problem in my system it was not working, now I am doing it in another system and this time I am getting mutant pdb file at least.