10 September 2015 5 6K Report

Some proposed cross-linking reagent for starch are sodium trimetaphosphate, epichlorohydrine and citric acid. Is there another reagent to cross-link starch?

More Fateme Irani's questions See All
Has anyone used deep learning for 2D/3D REGISTRATION ?

I am a Master student in Biomedical (Bio-electrical) Engineering and work on " 2D-3D registration ( CT and X-Ray) as my thesis. I created DRR from CT and want use CNN( Convolutional Neural...

19 February 2021 6,354 3 View

How to calculate DNA concentration of a sample with high concentration buffer?

I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...

23 January 2021 9,440 2 View

Can DNase1 degrade the plasmid (circular) DNA?

I had a sample of plasmid extracted from e.coli and wanted to check the amount of Host cell RNA impurity with a fluorescent dye. to do this, first of all, I treated the sample with DNase I. (NEB...

11 January 2021 2,608 3 View

VASP - Convergence Problem?

Dear All, I am new to DFT calculation, and I am using VASP. In a lot of my jobs, the forces are already smaller than the EDIFFG tag that I set in the INCAR, but, the jobs won't stop!!! The jobs...

26 December 2020 1,045 6 View

Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

Where can I find a Twitter dataset for Preliminary Flu Outbreak Prediction Using Twitter Posts Classification?

Where can I find a Twitter dataset for Preliminary Flu Outbreak Prediction Using Twitter Posts Classification?

07 December 2020 4,700 2 View

How to generate DRR (digitally reconstructed radiography)?

I am studying 2D and 3D registrations. I need help and guidance in producing DRR from CT images. Is there an article or program that fully explains how to generate DRR from CT images? . I want to...

22 October 2020 7,876 4 View

What are measurement criteria of bacteriophages in TEM pictures?

I've got the TEM pictures of my purified bacteriophage whose host is S. aureus. there are various shapes with tail/tailless (maybe a little tail). in order to place it in a family, I need the...

15 October 2020 5,261 5 View

What is the reasons of appearance exothermic peak and oxidation in DTA results of HEAs?

Hi I synthesized a CoCrFeMoMnNi High Entropy Alloy by the VAR method. After DTA analysis, the sample was completely oxidized and an exothermal peak was observed. Due to that, the structure is FCC...

18 August 2020 7,204 3 View

What Can I do if I have not normality in my large scale sample(1122)?

I have 1184 sample for my test. They are in 5 age group. all of my data does not have normality and variance of my groups are not equal. what I can I do? Can I use parametric tests?

07 June 2020 422 11 View

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Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

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What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

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