What is a simple method to measure isoelectric point of a virus?

More Tatiana A Demina's questions See All

What software do you use to extract local field potential data from continuous recording?

I have raw electrophysiological data records (no filters were applied) and need to extract LFP signals.

23 February 2024 4,514 2 View

¿Cuales fueron las Rivalidades Políticas entre Echenique y Castilla?

También mencionen por qué se dio.

13 September 2023 6,651 1 View

What other solvent than DMSO and Water can I use to dissolve an aqueous extract (lyophilized extract) to evaluate the antimicrobial activity?

My extract is not easy to dissolve. For example, I need a stock concentration of 500 mg/ml. I have used DMSO but I can only dissolve for 250mg/ml(thick or viscous) and in water at 125mg/ml(it is...

30 August 2023 2,670 0 View

What is the best method to homogenize mouse lung tissue for RNA extraction?

Thank you for any suggestions.

30 July 2023 3,229 3 View

How to calculate the homogeneity of two mixed powders with ImageJ?

Dear Researchers, Please advise me on calculating the homogeneity of two mixed powders with ImageJ by SEM images. Articles, guides, and real case studies are welcome. I want to automate the image...

28 May 2023 6,124 5 View

Need Europian parner for a EURIZION felloship for a project devote to the biodiversity of small saline groundwater fed ecosystems, who could help?

The Department of Analysis of marine ecosystems and anthropogenic impacts of the Ukrainian Scientific Center of Ecology of the Sea, where I work, is going to apply for EURIZON Fellowship and we...

02 May 2023 477 1 View

Does anybody know how to run DFT with the B97-3c functional in Gaussian16?

B97-3c has been proposed in B97-3c: A revised low-cost variant of the B97-D density func...

25 January 2023 6,892 2 View

What is a right way of sampling kernels with nonuniform natural mycotoxin (DON) concentration ?

I would like to use natural contaminated kernels to evaluate effect of physical parameters on natural DON mycotoxin and validate the process. However, there are 2 issues: a high variation in DON...

22 January 2023 739 1 View

Why PEG-precipitated virus sample is too viscous?

We have been using PEG-8000 (10%) and NaCl (0.5 M) precipitation for concentrating phage samples before ultracentrifugation and for some of them, we got a really viscous solution after PEG...

29 November 2022 7,087 2 View

E-prime extension for Tobii eye-tracker gaze-contingent design experience?

Hello all, I am currently working on a gaze-contingent design with E-prime 3.0 and Tobii Pro Lab using E-prime Extensions for Tobii. My design requires stimuli to start only when fixation of at...

16 November 2022 5,311 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?

01 August 2024 1,180 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

How does nonpoint source pollution affect ocean environment & why point source pollution is often easier to manage than nonpoint source pollution?

How does nonpoint source pollution affect the ocean environment and why point source pollution is often easier to manage than nonpoint source pollution?

01 August 2024 8,014 2 View

Sandip Chakraborty

You can please follow the links below to obtain certain valuable informations:-

1. http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2010.04663.x/pdf

2. http://www.jbc.org/content/190/1/55.full.pdf

3. https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314729545976/litdoc18117233p16-18_20110830220013.pdf

You can also download the pdfs of the files attached herewith.

Panduranga Rao

Viruses are made up of different proteins and nucleic acids. If you are looking isoelectric point for purifying this virus using chromatography, it is of limited use. Unlike proteins, viruses behave differently in ion exchage chromatography.