Hi Marcus & John,
Is there any idea/method to check whether the protein is coordinated by Zn?
thanks,
If you need real time Mn concentration use Special dyes that bind to Mn and can be assayed in fluorometer / fluorescent microscope. You can consult Molecular Probes (invitrogen.com) for it
If ypou want to quantify in in microscopic structures you can use both TEM and SEM electron microscopy with x-ray fluorescence set-up. You will get a spectrum of all metal concentrations in small specific structures but with a high lower limit of etection. Good for accumulated trace elements concentrations. For a very high sensitivity bulk analyses use the carbon-rod atomic-absorption spectrometry with a detection limit of part per billion of the specific element of interest
Flame detector, which is usually coupled with GC or HPLC, is the most sensitive to Mn2+ or any other metal detection in any sample.
Dear Raphael, Thank you for your input. Might you have more info on the microscopy methods or a paper reference? The high resolution provided by TEM would be ideal if it is sensitive enough. We are likely looking for micro molar Mn2+ above background. Warm regards, John
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