How to extract peak coordinates from a given cluster with MATLAB as in the SPM results viewer?

Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...

26 January 2021 7,672 2 View

What factors influence the growth of the informal economic sector?

Business in informality can be caused by factors such as the facilitating business environment, tax rates, forms of consumption by buyers, lack of administrative knowledge, complexity of the...

01 January 2021 7,189 5 View

Is there any index number for first author in journal article like h index?

In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?

15 December 2020 8,231 14 View

How to identify original journal website?

There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...

18 November 2020 7,650 6 View

If an observed d falls beyond the upper limit in equivalence testing, what does that mean?

I have been taking your Coursera Classes and reading some of your articles on equivalence testing, and I do not know how to interpret and effect and its corresponding CI, but I do not how to...

12 October 2020 9,046 5 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Appropriate stats test for multiple DV, multiple treatments, paired across days?

Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...

03 July 2020 4,487 7 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Is there any correlation to relate temperature and shear strength of Aluminum alloy AA2024-T4?

As shear strength of AA2024-T4 alloy is inversely proportional to the temperature. is there any equation to predict shear strength of AA2024-T4 with respect to temperature?

12 May 2020 8,804 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View