We have used HepG2 but nothing shows up in those lanes after ECL, and for qPCR the signals were very low, almost not detectable. Any recommendations?
Use beta actin as internal control
Hi Sana
With my Human Brain samples, I use RPL13( qPCR) and Beta Actin, GAPDH or B-Tubulin as internal control and they work fine for me.You could try any of this.
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