What is a good marker of mitochondrial health/function that can be used on retinal tissue?

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Pedro Escoll

In living cells, monitoring of mitochondrial membrane potential with dyes like TMRM by confocal microscopy (spinning disk) can be a good choice.

For IHC in fixed cells, staining of Cytochrome C can be an option.

Good luck!

Philippe D'Onofrio

Thanks very much, Pedro!

Sébastien P. Gendron

You can also check mitochondrial membrane potential with dye like JC-1.

Merci beaucoup, Sebastien!

Nicolas Cuenca

Cytochrome C is a good choose

Thanks Nicolas! I'm actually also interested in Cytochrome C degradation, do you know if it's possible to see fragments of it resulting from cleavage?