hi everyone, I am working on differentiation of fibroblasts to iPSCs. I am planning to. use matrigel coated plates, but am a little lost on what concentration I should use. I am using Corning matrigel (REF 356234). The batch I have has a protein concentration of 8.9 mg/mL. the data sheet just says to not dilute to less than 3 mg/mL. in your experience what isna good concentration to use for this application?
Dear Patricia,
in our lab we also work with iPSCs and use Matrigel. We dilute it to 5 mg/mL concentration as a working stock/aliquots. Since each lot has a different protein concentration, you have to calculate the specific volume of media needed each time.
This gets further diluted for each coating so that we end up with 200 µg per well of a 6-well-plate.
For a more detailed protocol you can have a look at this paper:
Generation of human iPSC-derived neural progenitor cells (NPCs) for drug discovery of neurological and mitochondrial disorders
(Zink et al., Bio-protocol, 2021) DOI: 10.21769/BioProtoc.3939
Good luck with your experiments,
Selene
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies