Patricia Ablanedo Morales

3 Questions 2 Answers 0 Followers

Questions related from Patricia Ablanedo Morales

Is my protocol effective for extraction of protein from cell fractions?

Fractionation 1. Scrape cells and collect by centrifugation (500 × g, 5 min, 4 °C) 2. Resuspend cell pellets in lysis buffer and incubate on ice for 10 minutes 3. Centrifuge at 500 x g for 5 min...

26 March 2024 6,314 3 View

Good protocol for fractionation and immunostaining of human fibroblasts?

Hello everyone I am looking for recommendations on a good protocol for fractionation and immunostaining of human fibroblasts.

31 October 2023 6,775 0 View

What is a good dilution to use to coat plates in matrigel that will be used for cell differentiation?

hi everyone, I am working on differentiation of fibroblasts to iPSCs. I am planning to. use matrigel coated plates, but am a little lost on what concentration I should use. I am using Corning...

18 January 2023 9,178 1 View