What is a good definition for "critical genes"?

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In your opinion, what are the basic skills necessary to a bioinformatician?

Inside the Computer Science and Biology fields.

01 February 2013 9,571 9 View

How do I get a gene list of fully sequenced and assembled genomes? (~9Mpb)

I want to perform a simple comparison test between 3 genomes, build a Venn diagram to analyze the percentage of common, exclusive and different genes among these 3 genomes.

31 December 2012 4,831 4 View

How to use order as profile level in STAMP?

I have my metagenome uploaded in MG-Rast. How can I download the data and use "order" (level) in STAMP to run statistical analysis?

11 December 2012 1,860 0 View

It's possible to have two substrate binding protein domains in a single ABC transporter?

I have found it in a bacteria genome during an analysis in slilico. Thanks, Merry Christmas and Happy New Year.

11 December 2012 2,482 4 View

How to compute differences among 2 entire and closed genomes?

I have 2 genomes fully assembled and annotated. (9Mb. each, aprox.) I want to do a basic analysis to discover difference (%). Nothing so complex, maybe align these 2 genomes and discover the...

10 November 2012 9,923 11 View

How to validate a hypothetical protein?

If I have a hypothetical protein in my genome sequence (bacterial genome sequence) and I want to do a lab study (not silico analysis) to validate this protein, what are the basics steps to goal?...

10 November 2012 8,223 2 View

Using STAMP to analyze metagenome data uploaded in MG-Rast.

I have 3 file types downloaded from MR-Rast. (1) *.FNA, (2)*.TXT, (3)*.RMA. How can I import to STAMP to analyze my data? I know its necessary a Profile and a Metadata archive in STAMP. How can...

10 November 2012 2,591 4 View

What is the difference between "sn-glycerol-3-phosphate" and "glycerol-3-phosphate"??

What means this "sn" means?

08 September 2012 6,469 9 View

What is the better way to express difference between two data tables?

Bar Graph? Pizza Graph? No graph? I have two data tables, but the numbers it's too similar between both, a LITTLE difference occurs in my data. I want to show this for the public. Show can i...

08 September 2012 9,514 5 View

Are ABC transporter sequences usually organized in cluster in genome?

In my genome I always found ABC transporters organized as side by side genes. Is it a pattern for ATP-binding cassette transporters?

07 August 2012 1,646 3 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View