What is a good definition for "critical genes"?

More Arthur Fernandes Siqueira's questions See All

In your opinion, what are the basic skills necessary to a bioinformatician?

Inside the Computer Science and Biology fields.

01 February 2013 9,528 9 View

How do I get a gene list of fully sequenced and assembled genomes? (~9Mpb)

I want to perform a simple comparison test between 3 genomes, build a Venn diagram to analyze the percentage of common, exclusive and different genes among these 3 genomes.

31 December 2012 4,799 4 View

It's possible to have two substrate binding protein domains in a single ABC transporter?

I have found it in a bacteria genome during an analysis in slilico. Thanks, Merry Christmas and Happy New Year.

11 December 2012 2,446 4 View

How to use order as profile level in STAMP?

I have my metagenome uploaded in MG-Rast. How can I download the data and use "order" (level) in STAMP to run statistical analysis?

11 December 2012 1,830 0 View

How to compute differences among 2 entire and closed genomes?

I have 2 genomes fully assembled and annotated. (9Mb. each, aprox.) I want to do a basic analysis to discover difference (%). Nothing so complex, maybe align these 2 genomes and discover the...

10 November 2012 9,881 11 View

How to validate a hypothetical protein?

If I have a hypothetical protein in my genome sequence (bacterial genome sequence) and I want to do a lab study (not silico analysis) to validate this protein, what are the basics steps to goal?...

10 November 2012 8,187 2 View

Using STAMP to analyze metagenome data uploaded in MG-Rast.

I have 3 file types downloaded from MR-Rast. (1) *.FNA, (2)*.TXT, (3)*.RMA. How can I import to STAMP to analyze my data? I know its necessary a Profile and a Metadata archive in STAMP. How can...

10 November 2012 2,535 4 View

What is the difference between "sn-glycerol-3-phosphate" and "glycerol-3-phosphate"??

What means this "sn" means?

08 September 2012 6,414 9 View

What is the better way to express difference between two data tables?

Bar Graph? Pizza Graph? No graph? I have two data tables, but the numbers it's too similar between both, a LITTLE difference occurs in my data. I want to show this for the public. Show can i...

08 September 2012 9,479 5 View

Are ABC transporter sequences usually organized in cluster in genome?

In my genome I always found ABC transporters organized as side by side genes. Is it a pattern for ATP-binding cassette transporters?

07 August 2012 1,605 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View