I'm starting with 1mg of BAPTA and trying to make aliquotes that can be used later to create 2mM BAPTA for flexstation experiments.
I'm interested in using baculovirus (BacMan system) mediated delivery during my project involving store operated Ca2+ entry (SOCE) and STIM/ORAI signal transduction. Baculovirus-transfection would...
05 June 2016 6,459 3 View
Hi, everybody. I'm preparing FRET AB experiment and at the moment my only available pair is YFP-RFP. I'm fully aware it is not common donor-acceptor pair as EGFP-YFP, CFP-YFP or CFP-RFP, however,...
31 December 2015 2,735 3 View
Hi, everybody. Currently, I am working with the cell cultures (HEK293 cell line). I'm looking for the most common (preferably most affordable dyes, too) that will efficiently label proliferative...
05 June 2015 5,930 4 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I want to work on medical image(disease) detection and classification. Where can I found Image data sets?
24 February 2021 3,130 3 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...
23 February 2021 3,411 4 View
I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...
19 February 2021 7,447 2 View
I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...
18 February 2021 4,499 1 View