Petra Visic

4 Questions 6 Answers 0 Followers

Questions related from Petra Visic

Can I use baculovirus-transfection when researching signalling pathway in HEK293, CHO and MDA MB 435s cell lines?

I'm interested in using baculovirus (BacMan system) mediated delivery during my project involving store operated Ca2+ entry (SOCE) and STIM/ORAI signal transduction. Baculovirus-transfection would...

06 June 2016 6,471 3 View

How to troubleshoot for FRET AB experiment where the only donor-acceptor available pair is YFP-RFP?

Hi, everybody. I'm preparing FRET AB experiment and at the moment my only available pair is YFP-RFP. I'm fully aware it is not common donor-acceptor pair as EGFP-YFP, CFP-YFP or CFP-RFP, however,...

01 January 2016 2,750 3 View

What is a good concentration of BAPTA in DMSO when making aliquots?

I'm starting with 1mg of BAPTA and trying to make aliquotes that can be used later to create 2mM BAPTA for flexstation experiments.

10 October 2015 3,187 0 View

What are the most common (affordable) dyes when examining cell culture proliferation/death?

Hi, everybody. Currently, I am working with the cell cultures (HEK293 cell line). I'm looking for the most common (preferably most affordable dyes, too) that will efficiently label proliferative...

06 June 2015 5,942 4 View