Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Could you suggest how can I count the floating point operations(flops) in the MATLAB codes?

Computational costs are important in numerical methods, as if they were obtained by counting the number of operations(flops). The order to count this number has been asked by MATLAB that I have...

22 August 2019 3,046 8 View

Calculating Potential of mean force (PMF) using SMD trajectories!?

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25 February 2019 4,297 1 View

How to interpret the surface roughness trend in PES/GO membrane?

adding graphene oxide / rGO / graphene have shown conflicting surface roughness changes in Polyethersulfone. for rGO in specific, why do we have roughness decrease whilst porosity has increased?

11 February 2019 1,354 4 View

Compatible forcefield with charmm to simulate a protein containing Trimethyllysin!?

Hi all, I am trying to simulate a protein containing a modified Lysin : Trimethyllysine. I am a NAMD user and I couldn't find a proper forcefield (topology and parameter files) with charmm yet....

02 December 2018 4,619 8 View

How should I define "non protein" parts of PDB in Parameter file(charmm)?

Hi, I need help with performing a simulation of a ligand and a protein. I am using psfgen code and add a ligand as the second segment but my parameter file couldnt recognize the new molecule...

20 September 2018 2,681 5 View

What is the best alghorithm to superpose two protein? Same in sequence vary in shape?

these two algorithms seems fast and efficient for aligning (superpose) two proteins: Acta Chrystallogr. Sect. A 61 (2005) 478 [doi: 10.1107/S0108767305015266] J. Comput. Chem. 31 (2010)...

18 September 2018 2,024 1 View

Does anyone know that can i use sudan black b instead of sudan 4 in En-face staining of whole aorta?

I don't have Sudan 4 hence I want to know I can use Sudan Black instead of Sudan 4

09 June 2018 5,028 3 View

Temperature control (via langevin) in Steered Molecular Dynamics!?

Dear all, I would like to know about the temperature control in Steered Molecular Dynamics. should I turn off langevin control in steered molecular dynamic? Thank you

05 June 2018 9,862 3 View

Why does my polymer solution tends to stick to the electrode while being imposed to electric field?

when I apply an electric field over my polymer solution it tries to move toward the negative electrode (the distance between the two electrodes is very low) this local movement is different every...

01 March 2018 6,984 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View