Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Could you suggest how can I count the floating point operations(flops) in the MATLAB codes?

Computational costs are important in numerical methods, as if they were obtained by counting the number of operations(flops). The order to count this number has been asked by MATLAB that I have...

22 August 2019 3,046 8 View

How to interpret the surface roughness trend in PES/GO membrane?

adding graphene oxide / rGO / graphene have shown conflicting surface roughness changes in Polyethersulfone. for rGO in specific, why do we have roughness decrease whilst porosity has increased?

11 February 2019 1,354 4 View

Compatible forcefield with charmm to simulate a protein containing Trimethyllysin!?

Hi all, I am trying to simulate a protein containing a modified Lysin : Trimethyllysine. I am a NAMD user and I couldn't find a proper forcefield (topology and parameter files) with charmm yet....

02 December 2018 4,619 8 View

How should I define "non protein" parts of PDB in Parameter file(charmm)?

Hi, I need help with performing a simulation of a ligand and a protein. I am using psfgen code and add a ligand as the second segment but my parameter file couldnt recognize the new molecule...

20 September 2018 2,681 5 View

What is the best alghorithm to superpose two protein? Same in sequence vary in shape?

these two algorithms seems fast and efficient for aligning (superpose) two proteins: Acta Chrystallogr. Sect. A 61 (2005) 478 [doi: 10.1107/S0108767305015266] J. Comput. Chem. 31 (2010)...

18 September 2018 2,024 1 View

Does anyone know that can i use sudan black b instead of sudan 4 in En-face staining of whole aorta?

I don't have Sudan 4 hence I want to know I can use Sudan Black instead of Sudan 4

09 June 2018 5,028 3 View

Temperature control (via langevin) in Steered Molecular Dynamics!?

Dear all, I would like to know about the temperature control in Steered Molecular Dynamics. should I turn off langevin control in steered molecular dynamic? Thank you

05 June 2018 9,862 3 View

Why does my polymer solution tends to stick to the electrode while being imposed to electric field?

when I apply an electric field over my polymer solution it tries to move toward the negative electrode (the distance between the two electrodes is very low) this local movement is different every...

01 March 2018 6,984 4 View

What are the techniques for adherence improvement between polymer thin film (water soluble ones) and metal surfaces?

I need to cover a metal surface with a thin layer of a water soluble polymer and this layer should adhere very well and not separate , also no-toxic ... what are the feasible methods?please...

30 January 2018 6,040 9 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View