I am looking for monoclonal antibodies specifically against the activated form of GNA12 protein. Has anybody come across any company that sells this particluar one?
I need to calculate the conductivity of the my material. From the previous reported articles i got these formulas 1. σ ∝ exp(-Eg/2*Kb*T) Kb - Boltzmann constant T - Temperature (in kelvin) Eg...
25 April 2024 1,421 3 View
I need to optimize the structure in solvent mixture of ethanol and toluene in various proportion (Gaussian 16 software) using the DFT methods. How can I define this in gaussian 16...
29 July 2023 1,458 3 View
Any kind of suggestions based on Sol-gel.
08 June 2023 7,033 1 View
Being an essential parameter, basal velocity estimation of a valley glacier is still not significantly discussed which helps in assessing the glacier water storage capacity over a period and...
02 November 2022 8,153 0 View
This is in reference to the diesel fuel specification with respect to China VI, Euro VI, BS VI and Wordwide Fuel Charter 2019 wherein the Flash point varies from 35 to 60 deg C. While flash point...
11 September 2022 7,682 1 View
Most of the studies related to the High mountain glaciers adopt a mass conservation approach at a global level while other approaches are least adopted.
06 February 2022 9,985 0 View
I have been trying to troubleshoot two issues,1) low yield of neurons 2) avoiding cell death after day 10 of culture. I'm trying to isolate mouse cortical neuron culture in a new lab from P(0-1)...
24 March 2021 3,910 4 View
I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...
13 February 2021 8,483 1 View
For 1st question, I am pursuing a Ph.D. program and have completed a Master's degree as the highest degree. For 2nd question, I can listen, speak, and write the English language. Kindly help me...
14 December 2020 6,176 3 View
I prepared a custom media with almost all essential components added individually (glucose, glutamine, all other amino acids, sodium pyruvate, sodium bicarbonate and HEPES), for an experiment. I...
29 March 2019 8,067 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View