PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

Confusion on selecting chinese option. Could you help me out?

For 1st question, I am pursuing a Ph.D. program and have completed a Master's degree as the highest degree. For 2nd question, I can listen, speak, and write the English language. Kindly help me...

14 December 2020 6,001 3 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

HEA Materials - reg I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir?

I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir. Thanking You. Regards J.Maya Research Scholar Advanced Materials Processing...

29 July 2018 1,738 6 View

Can anyone suggest a good software for simulation of half cut solar cells?

A standard solar cell is cut into four parts i.e. cut perpendicular to the busbar. The cells are then placed around 2mm apart and connected in series. I need a software that can help me achieve...

22 June 2018 4,125 5 View

What is the appropriate test to analyze significance in categorical variables?

I am analyzing physical activity (PA) data as part of a larger RCT study and I want to see if there has been an improvement in PA levels from baseline to post intervention between the intervention...

07 April 2018 1,015 3 View

Why does PBS treated with o-phthaldialdehyde exhibit absorbance at 332 nm when it is only a blank with no amine groups?

12 July 2016 8,655 3 View

I need to study about fat metabolism in liver cancer cell lines. What cell lines can be preferred from nccs, India?

Study is to check the expression of critical genes responsible for TG accumulation in hepatocytes, after inducing fat in the cell line

26 May 2016 4,041 1 View

Structure of protein complexes: using phase from one subunit to solve the other. Details?

When the structure of one of the subunit in a heterodimeric protein complex is known, can that phase be used to solve the structure of the other subunit for which no homology exists? In that case...

29 November 2015 288 2 View

Why when I extracted DNA from saliva using the salting out method is the DNA obtained very low (2ng/uL. 260/280 is 2.1 and 260/230 is 0.4)?

Please help me understand what is going on here. I actually extracted DNA from the same person's cheek swab and saliva. Four out of six samples generated only ~2.0ng/ul and the remaining two...

29 January 2015 8,795 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View