Confusion on selecting chinese option. Could you help me out?

For 1st question, I am pursuing a Ph.D. program and have completed a Master's degree as the highest degree. For 2nd question, I can listen, speak, and write the English language. Kindly help me...

14 December 2020 6,001 3 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

HEA Materials - reg I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir?

I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir. Thanking You. Regards J.Maya Research Scholar Advanced Materials Processing...

29 July 2018 1,738 6 View

Why does PBS treated with o-phthaldialdehyde exhibit absorbance at 332 nm when it is only a blank with no amine groups?

12 July 2016 8,655 3 View

Structure of protein complexes: using phase from one subunit to solve the other. Details?

When the structure of one of the subunit in a heterodimeric protein complex is known, can that phase be used to solve the structure of the other subunit for which no homology exists? In that case...

29 November 2015 288 2 View

What is space vortex theory and space power generator? Is it possible to get more than 100% efficiency from a machine?

Free energy

16 October 2013 1,742 7 View

What is the best method of validating miRNA expression in tissues?

We have compared the global miRNA expression profiling in spleens of diseased and treated mice. What is the most popular method in terms of validating miRNA expression among the following: probe...

19 November 2012 6,840 3 View

How would you confirm that a gene deletion by CRISPR is bi-allelic?

I am trying to perform a gene deletion by CRISPR using a pair of gRNAs. I use two pairs of primers, one pair that flanks the deleted region - the edited cells show a shorter band when compared to...

01 January 1970 3,611 8 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View