Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

HEA Materials - reg I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir?

I am a research scholar interested in HEA. I am in need of HEA materials. Can You provide me some materials Sir. Thanking You. Regards J.Maya Research Scholar Advanced Materials Processing...

29 July 2018 1,738 6 View

Why does PBS treated with o-phthaldialdehyde exhibit absorbance at 332 nm when it is only a blank with no amine groups?

12 July 2016 8,655 3 View

Structure of protein complexes: using phase from one subunit to solve the other. Details?

When the structure of one of the subunit in a heterodimeric protein complex is known, can that phase be used to solve the structure of the other subunit for which no homology exists? In that case...

29 November 2015 288 2 View

What is the best method of validating miRNA expression in tissues?

We have compared the global miRNA expression profiling in spleens of diseased and treated mice. What is the most popular method in terms of validating miRNA expression among the following: probe...

19 November 2012 6,840 3 View

How would you confirm that a gene deletion by CRISPR is bi-allelic?

I am trying to perform a gene deletion by CRISPR using a pair of gRNAs. I use two pairs of primers, one pair that flanks the deleted region - the edited cells show a shorter band when compared to...

01 January 1970 3,611 8 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Which are the most recent implications of ESL Academic Discourse in language teaching?

Language teachers at UTP (Language Department) are increasingly interested in the role of academic papers in the development of language (English) teaching practices. Research papers in Academic...

22 February 2021 1,388 4 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View