How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Is there a free software that could calculate electron density on a given atom?

Dear collegues, as stated - I'm looging for a soft that would calculate electron density on a given atom of a small molecules. Either for Ubuntu 18.04 or latest MacOS. I tried multiwfn, but it...

26 January 2021 4,060 3 View

Did anyone use spaCy multi-langage model (xx_ent_wiki_sm) to analyze Eastern Eurpean languages (e.g. Estonian, Slovakian, Croatian)?

I'm looking for morphological models that could used for stemming (NLP) in python for following languages: Croatian, Czech, Estonian, Slovakian.

08 December 2020 1,277 2 View

How much can online processing methods reveal about linguistic representation?

Hi, It's a common practice in psycholinguistic research to infer from online processing about linguistic representation. I'm particularly interested in bi- and multilingual speakers. A good...

11 January 2020 8,252 3 View

What is a suitable MS technique for protein AAA?

Hello, I am new to the field of proteomics and interested in analysing aa composition of protein product (mainly storage protein). Have access to aa analyser but due to high running cost I'd...

16 July 2019 9,034 4 View

Is separation of porphyrins on a hypercarb (PGC) column possible?

Hello, I am struggling with separation of porphyrins (i.e. 5,10,15,20-Tetrakis(pentafluorophenyl)porphyrin, protochlorophyllide, chlorophyll a and b) using a hypercarb (porous graphite carbon)...

28 January 2019 1,423 3 View

How many people I should engage as participants in longitudinal study to have a proper group after drop-outs?

I am preparing my longitudinal study on the process of forming the vision of own adult life in emerging adulthood. I want to conduct two measurements (8-12 month break) and finally have (in a...

20 January 2019 9,949 9 View

Is there any specific technique for mice brain fixative and histopathology slide process?

brain tissue slide process

14 January 2019 4,828 3 View

Vision of own motherhood - do you know any research on this topic?

I am looking for any research on vision of own motherhood in young childless women (adolescence, emerging adulthood, early adulthood). I am interested how young women see their future motherhood...

24 October 2017 7,622 5 View

Band ordering in VASP

Hello guys I am performing DFT calculations of some defect complexes in a large diamond supercell using HSE06 approach implemented in VASP. The 1-st BZ is sampled with gamma point only and...

05 July 2017 7,997 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View