I use C. elegans for experiments and one of my mutants has a complex substitution mutation. A part of the promoter, the gene and a large downstream region is substituted by a few bases. How does this process work? Can such an original substituted DNA region jump to another DNA region? We did a PCR to check for the mutation and find that our mutant has this mutation. However, if we use primers in both exons, there's no difference between wild-type and these mutant worms. Hence, this DNA is still in there somehow. Does anyone know what could be the reason and how to solve this?
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