09 April 2015 3 10K Report

Hi everyone! I am trying to build up a setup that can do flash photolysis experiments to study some Ca2+ activated processes of ion channels. i need a flash lamp/laser that can give a several microseconds high energy pulse to uncage Ca2+ from NP-EGTA or DMN, and i want to measure the Ca2+ signal during experiments. Can anyone give me some suggestion on what instruments are necessary to do this? it will be great help if you can provide the company, model, price of patch clamp amplifier (can Axon 200B work?), microscope and objective, flash lamp or laser, polychromatic light source, CCD or PMT.

I know many papers mentioned these, but I looking for some high performance-to-price ratio choices.

Thanks!!

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