Hi everyone! I am trying to build up a setup that can do flash photolysis experiments to study some Ca2+ activated processes of ion channels. i need a flash lamp/laser that can give a several microseconds high energy pulse to uncage Ca2+ from NP-EGTA or DMN, and i want to measure the Ca2+ signal during experiments. Can anyone give me some suggestion on what instruments are necessary to do this? it will be great help if you can provide the company, model, price of patch clamp amplifier (can Axon 200B work?), microscope and objective, flash lamp or laser, polychromatic light source, CCD or PMT.
I know many papers mentioned these, but I looking for some high performance-to-price ratio choices.
Thanks!!
A High Pressure Xenon Arc Lamp will do the work. You can also use a laser source but that would be probably a bit more expensive. the rest of the setup is exactly as a classic patch clamp rig with Ca2+ imaging capability. You can make it as fancy (more expensive) or as simple (less costly) as your budget can permit. The usual companies are known: Zeiss; Olumpus, Leika...etc. Of course, the Axon 200B could be used but any other amplifier could do as well.
Hi Dr. Fouad Lemtiri-Chlieh, Thanks for your professional help! According to your suggestion, I think i have found a suitable Xenon Arc Lamp supplier (Hamamatsu), and i have an idle Axon 200B in my lab so it's great when you say it works. THANKS!
Hi Jorge, Thanks very much for your help! Yes, xenon arc lamp is a good choice to do uncaging experiments, although UV laser can give much faster pulse (~5 ns), the patch clamp amplifier is a low-pass sampling system at < 20 KHz, so i will choose xenon arc lamp instead. and i found a xenon lamp can generate a UV pulse in several microseconds, that will help, THANKS AGAIN!
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