What happens if protein sample is loaded into a single well multiple times (ie load 10ul, turn on power, turn off power, load another 10ul, repeat)?

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Carlo Martins

I think that if you are not using precast gels, would be a good idea to use more stacking gels, so that the consecutive loads of proteins get stuck in the pre-analytical gel and do not cause the appearance of many more bands than intended.

More ideas?

Best with your experiment

Kanomi Sasaki

Thanks for the answer Mr. Martins. The sample was completed loaded before the dye band reached the separating gel. Still have to immunoblot so we shall see...