Kanomi Sasaki

2 Questions 4 Answers 0 Followers

Questions related from Kanomi Sasaki

Why are there no bands in my IP lanes?

I performed an IP to pull-down an endogenous protein A. When I immunoblotted with anti-protein A, the film only showed bands for the input lanes. The first chemiluminescence treatment was with...

31 August 2016 8,507 6 View

What happens if protein sample is loaded into a single well multiple times (ie load 10ul, turn on power, turn off power, load another 10ul, repeat)?

Water got into a sample while it was boiling, so I had to load a large volume of a dilute protein sample.

11 August 2016 6,936 2 View