What happends if you store your ligation reaction at room temp for too long? (Plasmid+vector+t7 ligase and buffer)?

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Dominique Liger

Hi there,

Apart from losing ligase activity I don't think much happen to the ligation product if the ligation mix is free of any DNA degrading activity.

Steingrimur Stefansson

..and it has no bugs growing in it.

Shamsher S. Kanwar

Ligase activity shall be lost and the plasmid & DNA may gedrade by nucleases activity of contaminating microbes!