Irena Muffels

2 Questions 1 Answers 0 Followers

Questions related from Irena Muffels

Is HOECHTS/PI staining (and then quantify) a good assay to determine the amount of dead cells of a whole population?

I want to treat fibroblasts with 24h MG132 and then look a the amount of cell death. However, I want to correct for the amount of cells in total, because they also prolfirate in 24hour. My...

11 November 2018 8,997 3 View

What happends if you store your ligation reaction at room temp for too long? (Plasmid+vector+t7 ligase and buffer)?

Room temp is 20 dgrs

09 September 2018 9,380 3 View