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We are writing an essay on climate change and its impact on indigenous people circumpolar north people, native nations, islandic, aborigenal of pacific and so on
16 July 2024 5,248 3 View
I would like to add "RIvista di Agronomia ISSN 0035-6034 to the RG journal list
06 July 2024 7,380 1 View
Hello to all members of this community. I am interested in making a publication in a scientific journal about the research I have done, the title of my research is "Project Management Model to...
16 April 2024 6,800 3 View
Hello everyone, for my master’s thesis I have to make a phantom in PVA. From research in existing literature, the PVA at my disposal is poor for my ultimate purpose (I have thus activated myself...
14 April 2024 7,855 1 View
Hi there, I'm setting a monitoring effort based on trap-cameras. I know that the selection of the batteries depends on many factors suh as camera-model, replacement possibilities, etc. and that...
17 November 2023 4,333 1 View
The previous question is not a file to be added by clicking the bottom "ADD"
30 August 2023 8,658 0 View
Hello everybody! Is there a way to extract the velocity arrows from HEC-RAS? Or reproduce them externally from the simulation results? Thanks for your kind and professional support
13 July 2023 5,629 2 View
how to find if and citations of my articles?
10 May 2023 1,831 4 View
Dear all, I am evaluating the Young's modulus of graphene via Molecular Dynamics simulation with OPLS-AA potential. The method I am using is a tensile test by "pulling apart the opposite rows of...
16 April 2023 7,788 1 View
I'm currently working with mouse peripheral blood, but several issues came out every time. Actually we are using as collection site the tail, blood is drip in eppendorf containing EDTA and...
12 March 2023 544 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View