By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View

Should studies published in predatory journals be included in systematc reviews?

I am conducting a systematic review of intervention, and i observed that one included study could determine the statistical significance of the final meta-analysis. Problem is, that article has...

13 April 2020 922 9 View

OCR system for phonetic (IPA) symbols?

I need to convert manually annotated IPA transcriptions into digital format. Does anybody know of an OCR system able to recognise IPA symbols (even just for English phonemes)?

25 March 2020 5,982 0 View

Is it possible to read the deformation field file produced by SPM during normalization?

If (x,y,z) are the voxel coordinates of a point of the brain scan before normalization, what is its position after applying the deformation field generated during the normalization?

19 February 2020 10,004 3 View

Bibliography on the effect of soils on nectar and sap?

10 September 2019 8,922 4 View

What is the correct phenol red permeability through Caco2 monolayer?

Dear all, I am performing Caco2 permeability experiments using transwell inserts and I am using phenol red permeability assay to verify the monolayer integrity. What is the maximum phenol red...

06 March 2019 6,726 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

How much ethanol do I need in extracting capsaicin from red bell peppers?

The capsaicin recovered will be tested against aphids.

04 February 2019 5,811 5 View

Is anyone aware of studies concerning geostatisics in the environmental field?

I'm looking for studies on environmental analysis carried out by geostatistics

16 January 2019 5,356 4 View

CVD adhesion on silicon substrates?

Dear all, I am currently trying to uniformly deposit organic semiconductor via thermal CVD at low pressure (around 10 Torr), but I sometimes get either no deposition or not homogeneous deposition...

21 October 2018 3,185 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View