Paolo Marangio

10 Questions 3 Answers 0 Followers

Questions related from Paolo Marangio

Why do titers and productivity of 3-hydroxypropionic acid (3-HP) produced in an engineered cyanobacterium are so low compared to E. coli?

Titers and productivity of 3-hydroxypropionic acid (3-HP) in engineered E. coli is in the orders of grams per liter, namely 71g/L and 1.8 g/L/h, as reported by Chu et al., 2015...

26 March 2016 6,368 2 View

How can we prevent E.coli from being "killed" by the recombinant protein or bio-molecule that we have engineered E.coli to produce?

This may sound stupid/trivial to some of you, but I am not quite clear on this. I will explain myself. Let's suppose that I have created a recombinant plasmid into which I cloned the gene coding...

18 March 2015 9,612 5 View

Why when undifferentiated ES cells or iPS cells are injected into a human or mouse do they generate teratomas containing tissues from 3 germ layers?

Why does this happen? Why can't the the ES cells just differentiate into the cells of the tissue into which they have been injected? Why all the 3 germ layers and not just one? Why do they...

17 January 2015 9,203 4 View

Why does long wavelength light (i.e. red and near infrared) minimize absorption and scattering by biomolecules compared to short wavelength light?

Hello I was watching an introductory video to two-photon microscopy and came across the concepts of Rayleigh scattering and absorption. As I did not understand these concepts entirely, I would...

24 September 2014 4,158 3 View

How can I insert statistical significance (i.e. t test P value < 0.05) annotations on top of my column bars on excel?

I am currently plotting some graphs on excel. I would like compare the expression of a certain gene in different conditions. I made my calculations on excel and ended up with a normal column...

23 April 2014 6,586 26 View

What exactly is a ribozyme and can you explain in general terms how I can design one that will prevent expression of specific proteins?

Question above

08 December 2013 6,121 0 View

Why do cancer cells stimulate vessels formation (angiogenesis) even if they are known to constitutively perform glycolysis regardless of O2 presence?

According to this article, "Angiogenesis is stimulated when tumor tissues require nutrients and oxygen" (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1993983/). But according to the Molecular Cell...

03 December 2013 2,936 5 View

Can anyone reccommend an online databse/website where I can find gene sequences of bacteriocins?

From this website I managed to find the name of the genes that encode the bacteriocins that I am interested in (http://bactibase.pfba-lab-tun.org/main.php). Where can I find the gene sequence...

18 November 2013 8,855 4 View

How are plasmid containing antibiotic resistant genes able to promote themselves so that they are passed on to other bacteria?

I am interested in how this actually works. Is the bacterium sort of "aware" of the fact that its plasmid contains antibiotic resistance gene and therefore passes the plasmid to the other...

02 November 2013 2,751 3 View

Program for analyzing differences in cpDNA sequences within individuals from same plant species (intraspecific study of genetic diversity)?

I am carrying out an experiment to look at the genetic diversity of individuals from this endangered plant species endemic to Madagascar (Xerosicyos tripartitus). We don't know if these...

24 June 2013 8,300 7 View