So, I'm synthetisizing some cationic porphyrins but I'm just unable to observe them in TLC, as they stay in the application point.
Do you have any suggestion about it?
I had succesfully used Silica with Acetonitrile:MeOH:KNO3 0.2 g/mL 7:1.5:1.5 for some of them, but with some others this and different proportions doesn't seem to woek.
I would be grateful for any advice :)
Thanks and have a good research!
Assuming you want to isolate? Then I would avoid HNO3. Organic acids such as, TFA, HCOOH or HOAc are easier to remove.
Your mobile phase isn't polar enough for silica. Trya 80:15:5 H2O:MeOH:HOAc, v/v/v.
Alternatively, you could try C18 plates using H2O:ACN or MeOH (25:75, v/v) with a little acetic acid.
Please look at the following below links which may help you in your analysis:
https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1015.2194&rep=rep1&type=pdf https://www.jstage.jst.go.jp/article/analscisp/17icas/0/17icas_0_i587/_pdf
Thanks
Mark kelm alternatively, you could try C18 plates using H2O:ACN or MeOH (25:75, v/v) with a little acetic acid.This sounds good could might work.
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