I have mite specimens stored at -80C for eventual extraction of DNA. They are "dry" - i.e. not in ethanol or any other buffer or preservative. Our -80C freezer has started breaking down, and needs to be switched off for repairs. Can I move my specimens to a -20C for about 2 weeks while repairs take place? And what effect will this have on the long-term integrity of the DNA? The oldest specimens have been stored for 5 years, and I envision I may still be storing them for another 2 to 3 years before processing.
There may be possibility of distortion of 3D structure of DNA when you are transferring from -80 to -20, then to -80. But if you are storing it regularly in -20 degree there will be no Tm difference will occur, and cot curve will remain intact, and 3D structure of DNA will have no problem.
I agree with Dr.Ravi Kant Upadhyay ·
better you gradually increase them from -80 to -20 and then keep on that temp till your experiment.
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