22 May 2018 1 3K Report

When fixing for the micronucleus assay, methanol/acetic acid/ringer fixation is used, followed by methanol/acetic acid without ringer. I was wondering what the function of the ringer was. Any reference to an article is welcome.

More Jeroen Vierstraete's questions See All
Can anyone help me identifying this organism that formed orange coloured scum layers on lakes and low running rivers in summer?

In September this year, I noticed an orange/green coloured scum layer in a slow-running river (see pictures) close to my hometown (Deinze, Belgium) containing green and orange clusters, as can be...

04 October 2023 9,499 1 View

Do NK-92 cells autolyze?

Hi there, Im trying to setup an NK-assay using NK-92 as effectors and K562 as targets. I use a 96-well round bottom setup with 5x104 targets and 1:1 till 80:1 ratio E:T. After 2-4 hours i measure...

13 February 2022 3,495 0 View

How many female union leaders are there at sectoral level in the garment industry?

I'm trying to find out how many female union leaders there are at sectoral level in the garment industry. There are, at least, two in Indonesia. But how many, if any, female leaders are there in...

11 October 2020 1,886 3 View

Does anybody know a data source for (European) vegetation spectral signatures?

Dear all, I am looking for a data sources that collects, stores and make available spectral signatures of vegetation (either crop or natural vegetation), preferably of European vegetation. There...

11 May 2020 4,665 7 View

What are the pro or cons of using Monkeysurvey for developing a survey? Any experiences?

I'm planning field research, including a survey, in Indonesia. I'm looking into monkeysurvey as a way to develop an online survey, but I'm wondering what experiences, good and bad, researchers...

11 August 2019 3,237 3 View

Is it possible to do a (moderation) subsample analysis when both the IDV and Moderator are dichotomous variables?

At the moment I am writing my Thesis. I want to test the moderating effect of industry relatedness on a divestiture - firm performance relationship using subsample analysis. However, when...

28 April 2019 6,206 4 View

Anyone also noticed strikingly greenish colored glass eels (Anguilla anguilla) this spring?

This spring we encountered some striking greenish colored glass eels (see pictures in attach). So far, this pigmentation is restricted to the neck region (just behind the head) and a lateral area...

27 March 2018 5,041 4 View

What is the source of these drawings to identify coleopteran families?

These drawings of beetle body parts are part of multiple pages of copies of some sort, and I would like to find the original publication of these illustrations. It seems to include tropical...

19 January 2018 9,913 2 View

Cloning: BP reaction of large fragment: only a smaller fragment is found?

I perform long range-PCR of a 9kb fragment and purify the 9kb band from a gel. However after doing multiple BP reactions, i keep getting a smaller 3kb fragment that is in my plasmid, and no 9kb...

29 November 2017 8,754 3 View

Tsai-Wu failure criterion for othotropic materials failure strengths in equibiaxial tension . Why no transitivity?

Hi all, I'm trying to understand the Tsai-Wu failure criterion for orthotropic materials. On the wikipedia page I found a way to calculate F12 however I don't understand an assumption they make....

07 November 2017 7,357 0 View

Similar questions and discussions
Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Can we add calcium carbonate to any media as an indicator for lactic acid producing bacteria?

As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...

20 July 2024 7,504 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

What is wrong with my input file?

im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...

16 July 2024 6,040 4 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close