What does one band of RNA electrophoresis gel (like this one) means?

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Is anybody experiencing problems with the Quantum™ Simply Cellular reagents from Bangs laboratories?

We cannot discriminate the brightest populations when coupled with FITC-labelled antibodies

14 May 2024 9,055 0 View

¿Se conoce el PIB relacionado con la minería de la eurozona?

Necesito información sobre el PIB relacionada con la minería de los países de la eurozona. Gracias.

14 February 2024 8,544 0 View

Does anyone have advice for adherent cells not attaching to multi-layer flasks when scaling up?

I have been culturing human microvascular endothelial cells (HMEC-1) on my own-made low-serum media. The cells expand fine in wells and flasks T25, T75, and T180 using 0.2 ml/cm2, so the media is...

23 November 2023 1,439 1 View

Could you please give me access to Europe-a community of values. Comtemporary underestanding of the idea and heritage of Alcide De Gasperi?

I am currently workingo on a essay on Adenauer, De Gasperi and Schuman and the construction of european unity

06 August 2023 4,063 1 View

Are you going to cancel my contract with Reseachgate by the end of March 2023?

I continue to be active in research. The information provided by Researchgate continues to be very useful for me. I would be very grateful if Researchgate does NOT cancel out my contract but, on...

16 March 2023 7,997 1 View

How can I convert a list of CAS numbers to SMILES in a .csv/.txt/.sdf file?

Hi world :) I need to convert a list of CAS numbers (~4000) to SMILES. Is there any workaround for it? I've tried some methods here but only one by one gave me success. A batch conversion would...

30 September 2022 9,681 0 View

How to benchmark a SPR setup?

I have built my own SPR setup and I wanted to test the overall design. It essentially contains all the necessary SPR components like the laser, glass with gold coating, and a detector to capture...

10 March 2022 187 0 View

Is there a way to couple light into fiber optic cable from the middle rather than the ends?

Many of the research papers I looked through have the laser or light source at one end of the fiber optic cable and a detector at the other end. But I wanted to know if there was any research on...

18 December 2021 8,354 14 View

What is the common coating thickness used for Fibre Optic SPR sensing?

I came across a Fibre Optic SPR sensor used with a phone and I was trying to figure out what thickness they used for their sensing...

13 December 2021 3,343 3 View

Is there an anti-mouse EGFR blocking antibody for in vivo experiments?

I know Cetuximab does not recognsize mouse EGFR and there is a publication demonstrating that 7A7 does not bind human or mouse EGFR (10.18632/oncotarget.24242). What other options are there in...

08 November 2021 9,338 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View