What does it mean to have multiple stop codons in mt-DNA sequences?

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How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

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How do I perform Electrochemical Impedance Spectroscopy with minimal errors?

Recently, I made a python code to fit the impedance data of perovskite solar cell I have gathered with a certain model of equivalent circuit. I used Nelder-Mead optimization but I think I need...

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What is the minimum level of knowledge about chemistry I should learn to study about perovskite solar cells as a physicist?

I am a master degree student wanting to learn deeper about perovskite solar cells. My knowledge about chemistry is rather half-baked. Please let me know the necessary textbooks I could learn in...

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Does anyone here know how to deposit in GenBank a small sequence of 66 pb of a variable domain II ompA gene of CT?

Is it possible deposit such a small DNA fragment sequence?

13 November 2012 6,817 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

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Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

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Does anybody have experience in expressing and incorporating two proteins (to express as a protein complex) in one cloning site?

I want to clone have two proteins cloned into pPROexHtb+ (which has one cloning site). I want to express them as a protein complex. My question is would you generally put a stop codon after first...

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Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

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Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

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Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

What is the possible biological explanation of the mutational pressure as a driving force on the evolution of particular species ?

In one analysis major role of mutation pressure was observed in codon usage bias of some species

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Can I make a nanoparticle with both magnetic moment and large g-factor Lande?

I am interesting in the SQUID coupled to the nanomagnet. It has been already shown [1] that the SQUID feels the nanomagnet nicely, but otherwise the nanomagnet does not feel SQUID, because it...

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Submission of dna sequences to NCBI?

When i submit dna sequences to NCBI, they ask for clarification saying "Some or all of the protein coding sequences contain internal stop codons, reading frame shifts (insertions/deletions...

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Norma Afiati

Please ignore this question since I cannot delete it. I found out the way to study and to understand it. Cheers.

Sara Karimi

Hi Norma Afiati super interesting question!! Could you write your answer? That would be great!

Randy Chance

Hello, how have you been able to get around this? Thanks Norma Afiati