What does it mean to have multiple stop codons in mt-DNA sequences?

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How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,682 4 View

What if BLAST result very much different to the morphological character of the species?

Dear Colleagues, I am seeking your experience about BLASt result. Having been following after the morphological character of a cephalopod specimen I identified it as Abralia andamanica, as also...

15 September 2019 5,800 5 View

To test a model prediction of behavior using SEM, is it necessary to measure the variables at 2 points-time?

Hello everyone, I am working on testing fruit and vegetable intake using the HAPA model and I'm planning to use SEM analysis. Looking at the literature, it is highly recommended that to test...

20 May 2019 1,498 0 View

How to annotate features for GeneBank submission please?

I have submitted COI subunit 5 of mitochondrial DNA from wildtype Cephalopods to NCBI GeneBank through BankIt. It seemed that I have completed the feature, alas these are the trimmed note for my...

13 April 2019 7,713 1 View

How do I perform Electrochemical Impedance Spectroscopy with minimal errors?

Recently, I made a python code to fit the impedance data of perovskite solar cell I have gathered with a certain model of equivalent circuit. I used Nelder-Mead optimization but I think I need...

18 August 2018 1,602 1 View

What is the minimum level of knowledge about chemistry I should learn to study about perovskite solar cells as a physicist?

I am a master degree student wanting to learn deeper about perovskite solar cells. My knowledge about chemistry is rather half-baked. Please let me know the necessary textbooks I could learn in...

01 January 2018 4,437 2 View

Radio-dating marine gastropod fossils?

Dear all, I have some marine gastropod fossils from Sangiran Central Java Indonesia which more than 75% of the collection were found in Recent/Quarter period; the sediment was classified as...

08 August 2016 5,366 7 View

How to perform protein extraction for differentiated 3T3-L1 cells?

These cells were differentiated in 24-well plate and I need to perform a western blot looking for a membranous protein (PARK7). I'm using the following procedure but it's not working. 1- 5 days...

03 May 2016 2,664 3 View

Does anyone know about egg axis in turtles?

Could you please explain about egg axis in marine turtle? How to position the egg in order not to change the axis when one have to move it to the artificial breeding ground? Can we see the axis...

15 December 2015 3,706 14 View

How do I block block obturator nerve lock using ethanol in children with spasticity?

Recently, phenol has been discontinued by the sole manufacturer in Canada. These obturator nerve blocks were previously done with phenol 6%. I have found some information on use of ethanol in...

15 June 2015 707 2 View

Should I put stop codon on light chain to form Fab?

If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?

20 May 2024 806 0 View

[LC-HRMS] Mass higher than normal for Intrinsicaly disordered domain?

For my project, I am attempting to purify an intrinsically disordered domain cloned in a pET28b vector. The strain used for overexpression is Rosetta II(DE3). Firstly, I encountered an unexpected...

23 April 2024 9,939 4 View

How to submit coding sequences in genebank?

Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It...

23 April 2024 2,468 6 View

In gene bank submission, what is the reason of "your seq. contains stop codons" ?

I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...

12 April 2024 3,503 0 View

Substitution matrix for codon (molecular evolution) ?

Hello, Does anyone knows how to get actual substitution matrices for codon ? I can get the amino-acid substitution matrices through the GiPhy website...

24 March 2024 5,614 0 View

Multiple primer sequences in sequencing result after PCR?

I was doing site-directed mutagenesis using PCR. And I have ordered forward and reverse primers that are reverse complements of each other (both primers are around 45bp, 7 aa before and after my...

19 March 2024 5,082 0 View

What should be the gc ratio of the genes to be expressed for pichia pastoris?

I need to know that after codon optimization what should be the GC content?

04 March 2024 7,074 1 View

Reference mapping and de novo algorithm for genome assembly?

Hello, I am working on viral metagenomics and have started using the High Throughput Sequencing reads for virus genome assembly. Since, I am at the beginner level, I have some confusions. As these...

01 February 2024 296 1 View

Cloning with pAcGP67A vector with GP64A signal peptide?

Hello everyone, Is there anyone who has cloned with pAcGP67A secretion vector with GP67A signal peptide. I tried to clone with insert 1.2kb in the vector, MCS: BamHI and NotI. C-term Thrombin and...

12 December 2023 5,091 1 View

Bacteriocin expression in pET-19b?

I am looking for vectors for expression of bacteriocin, and from pET series I think that 19b fits the most, I am just having few questions. When I clone my insert in NdeI and XhoI, i will have...

12 December 2023 2,321 5 View

Norma Afiati

Please ignore this question since I cannot delete it. I found out the way to study and to understand it. Cheers.

Sara Karimi

Hi Norma Afiati super interesting question!! Could you write your answer? That would be great!

Randy Chance

Hello, how have you been able to get around this? Thanks Norma Afiati