I have submitted COI subunit 5 of mitochondrial DNA from wildtype Cephalopods to NCBI GeneBank through BankIt. It seemed that I have completed the feature, alas these are the trimmed note for my submission:
Complete feature annotation has not been included for some or all of the sequence(s) you have submitted.
A. Please resubmit your sequence(s) with relevant features such as:
- coding regions (CDS features), partial or complete, including nucleotide spans and reading frame. Using this information, our software will add the amino acid translations for you. - structural RNAs such as rRNAs, tRNAs, misc_RNAs, with nucleotide spans - features which may describe your sequence, such as repeat_regions, UTRs, promoters with nucleotide spans.
B. If you are unable to determine the feature annotation, please provide a biological reason for the lack of annotation. Alternatively, you may choose to unverify your records and the following comment will be added:
COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter.
The commented record(s) will indicate that the data are unverified and your sequence(s) will not be included in NCBI BLAST databases.
Please analyze your data prior to resubmission. In order to avoid delays in receiving your accession numbers, ensure that your submission DOES NOT have:
-internal stop codons or reading frame shifts in any coding regions -sequences less than 200 bp -sequences containing vector contamination -untrimmed low-quality sequencing read ends
In addition, include: - unique identifying information for each sample, such as strain isolate, clone, specimen voucher or laboratory identifier - properly fielded source information using the correct source modifiers.
Could any one please explain? thank you.
Using sanger sequence data, this is an example of what I have submitted:
LOCUS Seq1 657 bp DNA linear INV 09-APR-2019 DEFINITION organismUroteuthis chinensis JC1 cytochrome oxidase subunit I (COI) gene partial cds mitochondrial. ACCESSION VERSION KEYWORDS . SOURCE mitochondrion Uroteuthis chinensis ORGANISM Uroteuthis chinensis Eukaryota; Metazoa; Lophotrochozoa; Mollusca; Cephalopoda; Coleoidea; Neocoleoidea; Decapodiformes; Teuthida; Myopsina; Loliginidae; Uroteuthis. REFERENCE 1 (bases 1 to 657) AUTHORS Afiati,N., Subagiyo,S., Hartati,R. and Kholilah,N. TITLE Direct Submission JOURNAL Submitted (09-APR-2019) Aquatic Resources Management, Universitas Diponegoro, Kampus Tembalang, Semarang, Central java 50275, Indonesia COMMENT Bankit Comment: LocalID:Seq1. Bankit Comment: BankIt2211899.
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..657 /organism="Uroteuthis chinensis" /organelle="mitochondrion" /mol_type="genomic DNA" /host="normaafiati123" /specimen_voucher="JC1" /db_xref="taxon:78424" /country="Indonesia: Karimunjawa, Central Java" /lat_lon="5.8658 S 110.4004 E" /collection_date="11-Aug-2018" /collected_by="Norma Afiati" BASE COUNT 196 a 121 c 100 g 240 t ORIGIN 1 acattatatt ttatcttcgg aatttgagca ggattagttg gtacttcatt aagattaata 61 attcgaacag aattaggtaa acccggatca ttattaaatg atgatcaact atataacgta 121 gtggttactg ctcatggttt tattataatt ttctttatag ttatacctat tataattgga 181 ggatttggaa actgattagt tcctctaata ttaggtgctc cagatatagc ctttcctcgt 241 ataaataata taagattctg attgcttcca ccatcactga cattattact agcttcctct 301 gcagttgaaa gaggagctgg aacagggtga acagtatacc cccctttatc cagtaatctt 361 tcccatgcag gcccttcagt tgacctagct attttttccc tacatttagc tggtatctca 421 tccattttag gtgccattaa cttcattaca actattataa atatacgttg agaaggatta 481 ttaatagaac gaatatcact atttgtgtga tctgtattta ttacagcaat tttattactt 541 ctttcccttc cagtactagc tggagcaatt actatacttt taaccgaccg aaactttaat 601 acaacatttt tcgacccaag aggtggtgga gatccaattt tatatcaaca tttattc //
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