How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View

Why would looking at the expression of mecp2 in the prefrontal cortex in rett syndrome be useful?

I don't believe there's much research in this area in humans. There are mice and primate studies

05 December 2020 4,556 2 View

Kinetic modelling of oxidative dehydrogenation of ethane ?

According to below articles about kinetic modelling of ODH reactions by G. Che-Galicia and et al. there are some problems need your guidance and advices: Kinetic and reactor performance of a...

27 November 2020 9,035 3 View

Interpretation of HSV 1 and 2 Immunoflourescence ?

Hi I am M.Phil researcher in a Pathology Lab. We have purchased the SimFlour HSV 1and 2 IF kit from a reputable company. We applied it on cervical smears and cell blocks prepared from SurePath...

06 November 2020 530 1 View

What are your experience with 96-Transwell formats?

I am planning to use a 96-well format for an experiment as this will greatly improve throughput and expedite treatments. I will be using Caco-2 cells cultured apically. I need them to form...

02 November 2020 5,287 3 View