What does it mean that the charge value of electrons can be accurately calculated with an error of less than 0.08% through three basic physical constants: vacuum dielectric constant, vacuum speed of light, and Planck constant?
That means that the contributions of the measurement errors of each of those constants add up to 0.08 %.
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
Where could I buy a magnetic shield layers which suppresses the magnetic field below 1μG?
26 April 2024 1,630 0 View
This is 12% SDS PAGE gel and the MW of this protein is around 20 kDa. Running: 80V 20min; 110V 2h30min Transfer: 100V 1h20min I wonder this might be due to the gel over-heated during the running?
04 January 2024 7,414 0 View
The cDNA was amplified by Q5 and PCR product was analyzed by agarose gel electrophoresis. Would cDNA template degradation cause such smeared or smiling bands?
13 December 2023 3,031 12 View
Hi everyone, I got a question about the methods/assays I can use to detect Riboflavin inside the cells. Also after treatment with RF ? Fluorescence spectroscopy, HPLC, ELISA, seahorse??? If...
02 December 2023 3,001 0 View
Pull down assay showed a discrepancy of band size between endogenous and over-expressed stable clones. Left: Endogenous Right: OE
30 October 2023 643 6 View
I used KAPA hifi pcr kit to linearize the plasmid for in-fusion cloning. Following the protocol, the DNA template amount was 5 ng and I already diluted it 100x. Annealing temperature: 66 69. The...
29 May 2023 5,232 5 View
Hi, dear friends online. I am a fresh person in bioinformatics with little understanding. but I need to learn to analyze RNA-sequencing data. I think python is simple, I want to use it to...
05 April 2023 2,626 3 View
Hell everyone. Recently I am modeling a logistic regression. The outcome Y has 40 subjects but only 10 events (Y = 1). I hope to figure out whether predictor A is associated with Y after...
22 February 2023 8,329 5 View
Hello everybody, I'm using cluster-mean standardized repeated measures data (intensive longitudinal data) in multilevel models. However, I only used part of the data in the models (e.g., the...
10 January 2023 4,439 4 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
07 August 2024 5,343 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View