What does a 55kDa protein indicate when on SDS-PAGE it runs as described below?

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Goritsa Rakleova

It means most probably that your protein aggregate after protein sample boiling. You should centrifuge the protein sample after boiling to get rid of the formed unsoluble aggregates ...

Adam B Shapiro

Your protein is aggregated. Boiling in SDS broke up the aggregates into monomers.

Lakshmi Prasoona Thota

Thank you!!