4 Questions 11 Answers 0 Followers
Questions related from Lakshmi Prasoona Thota
Hi there, Parasites are separated from RBCs using saponin lysis buffer and then parasite pellet is resuspended in RIPA buffer or any cell lysis buffer with protease inhibitors followed by...
07 July 2017 1,154 3 View
The regulation of tRNA genes in pACYC based codon plus RIL or in pLysS plasmid is unclear. Does anyone know what promoter controls the expression of these genes?? Does anyone possess the sequence...
04 April 2016 1,873 1 View
Hi, Has anyone tried or aware of purification of P.falciparum proteins from the infected RBC culture lysate (endogenous protein and not any tagged version or heterologous expression) thank you
03 March 2016 9,119 2 View
A 55kDa protein when run on SDS-PAGE in the presence or absence of reducing agent runs at monomer mass when boiled however it hardly enters the resolving gel when not boiled in presence or absence...
01 January 2015 7,956 3 View
