Qualitative Analysis Software Recommendations?

I am looking for expert recommendations on qualitative analysis software. I have been engaging in more mixed-method work recently and I need reliable qualitative analysis software. Any...

02 February 2021 7,984 7 View

How to tackle autofluorescence using the technique FISH in mouse frozen tissue?

Hi there, I am looking for some advice with FISH. This is my first time attempting this technique and I am not too sure where I could potentially be going astray. I have followed FISHTag RNA...

23 September 2020 3,849 1 View

What are the best emotional intelligence scales that contain subdimensions?

We are looking to do a study on leadership and we want to use a an Emotional Intelligence Scale that meets the following criteria 1. is used in published academic research, not just consulting...

14 July 2020 7,122 2 View

Stability of N-Benzyl substituted indazoles?

I am working with N-Benzyl substituted indazole-nucleus but I'm having many problem with the stability of the molecules. Does anyone know if the benzyl position is sensitive to something, such as...

29 June 2020 7,505 3 View

How to normalize a Raman spectrum of graphene oxide to calculate ID / IG?

I am normalizing with respect to the G band, in this way I obtain as Raman intensity for this band a value of 1.00 and for the D band a value of 1.42, in this way I calculate ID / IG = 1.42, I...

21 December 2019 6,923 3 View

CAMKII-alpha triple immunofluorescence help in Primate?

I'm having a heck of time! I'm trying to perform triple immunofluorescence for CAMKII (alpha; mouse; milipore 05-532; 1:5K; visualized with AlexaFluor 647), Fluorescein (Invitrogen, A11095, goat,...

29 October 2019 7,063 2 View

Phosphorylated and non-phosphorylated protein X in the same western via IR fluorescence secondary?

The size of protein X (Phosphorylated and non-phosphorylated) will be pretty much the same on a western. Let's say after primary antibodies incubation of the two separate proteins (Phosphorylated...

16 September 2019 5,583 5 View

Oil Red O staining protocol - best mount to use?

Hi, I am having trouble with my Oil Red O stain for my rat MSCs. What is the best aqueous mounting medium to use? I followed the protocol from IHCWorld...

03 April 2019 10,012 3 View

Statistical Method for Treatment Outcome Research?

Hello all, I am completing a paper on the change in impairment over the course of treatment. I am not only interested if seeing that there is change overall, but where the biggest change occurs...

24 February 2019 3,709 4 View

Quantification of osteoblast staining?

Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...

02 February 2019 7,584 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View