What do you mean by Heterologous Expression of gene? How you enhance the higher level of gene expression?

04 April 2016 1 3K Report

Now i am starting a project. so, please give me your valuable instructuins and suggestions. Thanks

Hi there,

It means using an host organism as a factory to produce a protein originating from an other organism. Usually the most used host organism is bacteria Escherichia coli which can be used for bacterial protein as well as for eukariotic protein provided you have the corresponding cDNA in hand for cloning.

Badges
Science topic

More Md Ekhlas Uddin's questions See All

What are the roles of presentation in scientific conferences ?

Roles of presentation in scientific conferences.

10 November 2018 4,338 0 View

How I pursue my expected higher degree PhD under any reputed Lab in any foreign country?

I pursue my expected higher degree PhD under any reputed Lab in any foreign country.

08 September 2018 9,095 3 View

How you are Exploring the molecular mechanisms for the interactions between marine algae and bacteria?

02 March 2016 9,200 2 View

How you are Investigating the carbon flow from marine algae to bacteria?

02 March 2016 905 2 View

How can i extract/isolate specific gene from Bacteria and clone it?

How can i extract/isolate specific gene from Bacteria and clone it? which methods are appropriate for above issue?

10 November 2015 597 0 View

How i get clear single band in SDS-PAGE?

How i get clear single band in SDS-PAGE? Please help me for your clear discussion. I am looking forward for your kind help, suggestion and cooperation in this regard

09 October 2015 8,434 4 View

How I can get an ISSN number for our newly establish an open access International Journal ??

We are establishing an open access International Journal. So, need an ISSN number with indexing process. So, please help me.

01 January 1970 760 7 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View