How can i extract/isolate specific gene from Bacteria and clone it?

Md Ekhlas Uddin @Md_Uddin78

11 November 2015 0 603 Report

How can i extract/isolate specific gene from Bacteria and clone it? which methods are appropriate for above issue?

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Md Ekhlas Uddin's questions See All

What are the roles of presentation in scientific conferences ?

Roles of presentation in scientific conferences.

10 November 2018 4,338 0 View

How I pursue my expected higher degree PhD under any reputed Lab in any foreign country?

I pursue my expected higher degree PhD under any reputed Lab in any foreign country.

08 September 2018 9,095 3 View

What do you mean by Heterologous Expression of gene? How you enhance the higher level of gene expression?

Now i am starting a project. so, please give me your valuable instructuins and suggestions. Thanks

03 April 2016 3,004 1 View

How you are Exploring the molecular mechanisms for the interactions between marine algae and bacteria?

02 March 2016 9,200 2 View

How you are Investigating the carbon flow from marine algae to bacteria?

02 March 2016 905 2 View

How i get clear single band in SDS-PAGE?

How i get clear single band in SDS-PAGE? Please help me for your clear discussion. I am looking forward for your kind help, suggestion and cooperation in this regard

09 October 2015 8,434 4 View

How I can get an ISSN number for our newly establish an open access International Journal ??

We are establishing an open access International Journal. So, need an ISSN number with indexing process. So, please help me.

01 January 1970 760 7 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View