16 January 2024 1 2K Report

I am aligning mtMutS octcoral specific gene sequences and i have read that gaps/ indels (9 base) within the sequences.

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What is the role of Disodium hydrogen phosphate in DAB staining?

Hello RG friends, I have stained the pathogen infected plant leaves with DAB staining solution for confirming the H2O2 (hydrogen peroxide) accumulation. Successfully I got the expected result....

18 December 2022 4,054 0 View

How can I calculate Colony forming unit (CFU)from a agar plate spread with crude extract?

I made the agar plate spread directly with the crude extract instead of doing serial dilution. So how can I calculate CFU from the agar plate? Or it can only be calculated from the serially...

05 February 2022 4,253 6 View

Why gyrB gene is used for the amplification of specific microbial sequences ?

I am working with phytopathogens. For the molecular identification, I need to amplify my bacterial DNA with primer. Usually, 16S rRNA is used as a universal primer. But for my bacteria, some of...

19 December 2021 1,032 4 View

Can anyone suggest the experimental procedure for heavy metal analysis in wastewater using ICP-OES?

Mainly dairy wastewater and fish wastewater

21 November 2021 4,425 6 View

The best antifungal agent?

I want to culture a specific bacteria on Nutrient Glucose Agar..so need to inhibit or suppress the fungal growth contaminations on that medium... please suggest me which is the best antifungal...

13 October 2021 5,660 8 View

How to retrieve temperature dependent results from abaqus?

Hi all, I am modeling a connection model in Abaqus using the component-based model. I have temperature-dependent properties of the elements in my model. It is running successfully but I am not...

01 April 2020 1,715 0 View

Human Mono-Macro Differentiation?

I realize this is a question that has probably been previously addressed! We are currently trying to differentiate monocytes to to macrophages using autologous serum from the same donor. The...

26 March 2018 5,402 4 View

What are available alloy steel materials whose hardness is less than H.S.S.?

I want to perform my research work on CNC lathe by varying cutting speed, feed, doc, and rake angle for opimum conditions of vibration and max MRR for using H.S.S. tool material and alloy steel...

02 December 2014 6,989 3 View

What are the differet metrics that can be used determine image quality perceptually?

I am working on Stereoscopic DIBR. I needed some metrics that can help me to compare rendered images. I am currently using SSIM. But I came across Peak signal to perceived noise ratio (PSPNR). Has...

10 November 2013 6,172 6 View

How i can do harmonic analysis in ansys work bench of miiling cutter?

modal analysis was done and natural frequency and mode shape available

01 January 1970 2,153 3 View

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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What is the acceptable p-value cutoff for GO enrichment analysis ?

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