What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Can anyone help me troubleshoot QPCR for AAV titering?

So the lab that I work in generates a lot of AAVs and we titer them via QPCR. However, I've been noticing that the entire time, the efficiency has always been very low (anywhere from 40-80%) with...

22 November 2020 4,580 3 View

Can plant tissues be stored at -20C for DNA extraction?

Hey everyone. I collected some leaf tissue samples from the plant Phragmites australis from which I am hoping to extra DNA for sequencing. I will be extracting DNA using Qiagen DNeasy Plant Mini...

08 November 2020 4,989 12 View

Does anyone know of a marker expressed on all lymph-resident cells that can be used in flow cytometry?

I am looking for a marker found on all cells that reside in the lymph node - I study the immune response in salivary glands and we want to gate out any lymph resident cells to ensure that there is...

13 October 2020 6,322 2 View

Can you expect to see changes in secondary structure in a MD simulation?

While running a simulation on any peptide or protein, is it possible for the secondary structures to change? For example, seeing a steady rise in helical content over the whole simulation?

30 September 2020 4,298 9 View

What are ideal personal desktop hardware specs for comparing models for time series analysis and machine learning?

I am researching trading strategies, particularly involving time series analysis, but also machine learning, on data sets that range as far back as 1950 and would like to compare the accuracy of...

15 July 2020 7,733 3 View

How do I determine if my Fst values are significantly different from 0 or between populations that are and are not separated by a highway?

I am trying to determine if a highway is too much of a barrier for shrews to cross and breed freely. What I have to work with is ~1080 bp of the mitochondrial cytochrome b gene (we did not do...

22 June 2020 7,647 1 View

What is the best wearable EEG recording device for research?

Hi there, I am searching a wearable EEG device with as many channels and the highest accuracy possible. I found some papers which reviewed/used the following devices, but still not sure which is...

19 May 2020 5,712 2 View

Help needed with the anatomy of a mouse knee. What tissue is this?

Hi all, I have been collecting cross-sections of a mouse knee joint, and I keep seeing some differently coloured, yellowish tissue on the anterior side of the joint, but I'm not too sure what this...

27 January 2020 5,792 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View