What cytotoxic compound could I used to produce a positive control for a WST-1 assay?

19 January 2017 1 3K Report

I am going to perform a WST-1 assay soon and I wanted to have all of the appropriate controls ready. From another post, I read that it would be better to have a positive and negative control. For cell viability, what cytotoxic compound can I use? I was thinking about 30% hydrogen peroxide, but I am not sure that would work. Any suggestions?

Thanks!

Max Hansen

We used 70% EtOH + 1% Triton X. I worked quite fine for our controls in XTT assays. H2O2 will kill your cells too, but i'm not sure if it will interfere with our assay.

Good Luck.

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