Cross linking is the most promising technique in material science for developing and modification of materials. Bio-material is the hot topics today in fields of biology and chemistry.
I am searching for the cross-linking agents which can be used invitro or invivo. Most of the crosslinking agents are cytotoxic and cause cell death. If anyone knows the crosslinking agents which can be employed invitro and invivo, kindly provide me information regarding this. Thanks
One but not exclusive example is "meat glue" http://en.wikipedia.org/wiki/Transglutaminase
I would highly recommend you to read Greg Hermanson's Bioconjugate Techniques book. All of your questions will be answered in that book.
Click chemistry is one of the modern technology for crosslinking agents
Reference
http://www.worldofchemicals.com/14/chemistry-articles/click-chemistry-applications-in-drug-discovery.html
maybe Glutaraldehyde with low concentration (less than 2%) can be the good one
You may also try the homobifunctional imidoester dimethylpimelimidate (DMP; spacer lenght of 0.92 nm) or the homobifunctional N-hydroxysuccinimideester bis(sulfosuccinimidyl) suberate (BS3; spacer lenght of 1.14 nm)
Can try physical methods of influence. We experimentally found in our studies that the gamma rays and radiation, have a "cross-linking" effect. They leave no residual radiation, able to "accumulate" the effect and have no damaging effect on tissue and cell. Naturally it is a "reasonable" use of radiation dose.
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