Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Thawing or defrosting methods for frozen soil samples?

I have soil samples that have been frozen for a while and I need to re-acclimate them for a series of analyses that require incubation at 25C. I have been looking for laboratory methods but seem...

04 January 2021 4,862 3 View

How to measure protein prescence in a tile scan in ImageJ?

Hello all, I have attached an image in which I would like to know the IntDen and/or the amount of protein particles. The wells are custom made, thus looking slightly warped sometimes. So far my...

10 February 2021 3,478 3 View

Global parameter in Abaqus?

Hello, im encountered on a problem in Abaqus that i cant solve. Setting: Parameter analysis in Isight include Abaqus model. The Model is a simple shaft hub connection with a standard contact...

26 January 2021 3,349 2 View

What should be the inner radius for practice coaxial cable mm wave antennas ?

I am trying to make a patch antenna for 28GHz and THz waves, but I m having confusion with the dimension of coaxial feed. wherever I find coaxial cables for millimeter dimensions, only the outer...

21 January 2021 7,119 2 View

Is there a solution to this conundrum on freefall into a black hole?

18 January 2021 1,582 3 View

Possibility of using a reference frame to account for dark matter, or summing the kinetic energies in a galaxy?

Dark matter is some extra energy that we observe which bends spacetime such that a star orbiting a galaxy will appear to have escape velocity but will remain in a stable orbit. When solving for...

20 November 2020 1,350 4 View

How can I determine the interaction radius (R0) of a polymer in terms of their Hansen solubility parameters?

I have two questions: (1) I want to analyze the miscibility between two polymers: poly(lactic acid) PLA and poly(glycerol sebacate) PGSeb. However, I haven't found any information about the...

06 October 2020 211 3 View

What is the most appropriate searchlight radius for a single-trial, MVPA analysis for a category learning task?

Hello everybody! We have a categorization task which loads heavily onto procedural learning systems, but we're looking to see if a manipulation we introduced recruits any additional neural...

14 September 2020 3,490 2 View

Simulation of compression testing of a spring?

Hi. I am doing simulation of compression testing of a spring.My experimental and simulation results are same in the linear region. I have tried the simulation with all types of contact behavior...

10 September 2020 1,746 3 View

Why does abaqus give wrong temperature results?

Hello, I made a simulation based on a cubic piece pressed against a rotating cylinder. when the cylinder has a small radius (1 cm), I am getting accurate temperature results. however, when I...

16 August 2020 6,507 4 View

What is the optimal opening (rim) angle of a solar parabolic (dish) concentrator ?

23 July 2020 3,403 3 View