Something is wrong with my Raman spectra of TiO2, Carbon dot and CD TiO2 composite. What can be the reason?

I have already taken XRD and FTIR for these samples which are fine. Pls check attachment.

20 September 2020 7,351 7 View

How to use both right and left axes of a graph in HFSS?

I want to plot both efficiency and gain in the same graph. How can make right side vertical axis as a gain and left side vertical axis as an efficiency (%) or vice versa?

22 July 2020 1,502 3 View

I am planning to express my protein in Pichia and would like recommendations on the best expression kits or individual components for expression?

I have no prior experience with expression in Pichia. I would like recommendations on individual Pichia cells and vectors, or expression kits. I also want to make decisions about secreted /...

19 July 2020 1,308 1 View

How to test MIMO antenna on vector network analyzer(VNA)?

The designed antenna is 2X1 MIMO antenna. I want to test its return loss on VNA. How to apply input to MIMO antenna? Shall I test one antenna at one time or both antennas should be excited at the...

24 September 2019 7,702 8 View

Is pET21 vector compatible with Rosetta host cells?

pET21 contains ampicillin gene as the selectable marker and Rosetta cells require Cloramphenicol because of the pRARE plasmid? What antibiotic should I use then?

16 May 2019 4,264 3 View

Which post hoc test do i choose for analysis of data from trials to study the effect of bacteria on rice growth and yield?

I am studying the effect of bacteria on growth and yield of rice. I have carried out pot trials and field trials for the same. I have done ANOVA in SPSS software. Which post hoc test should I...

27 March 2018 5,258 3 View

Why Sample in SDS-PAGE and MALDI TOF show different molecular weight ?

My protein shows single band at 28Kda in SDS-PAGE but for MALDI it shows a single peak at 23 Kda what could possibly be the reason

08 June 2016 999 4 View

How does one determine therapeutic potential of a nuclear hormone receptor?

When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...

10 July 2014 3,040 1 View

Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...

22 May 2014 4,701 3 View

How can I do dissociation of antigen bound to antibody on Protein G sepharose column?

I want to dissociate virus bound to antibody onto the Protein G sepharose high performance columns. Can anyone suggest a simple protocol which will enable me to separate them while keeping them...

19 May 2014 5,788 4 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View