What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Native gel blot unable to to observe antibody binding?

Hello, I'm trying to utilize a native gel for a protein dimer experiment. Everything seemed to work relatively well until I try to examine the blot in a ChemiDoc MP. I don't see any antibody...

30 September 2020 6,059 3 View

Isolates that grow on CHROMagar col-APSE will not grow on MacConkey agar supplemented with Colistin Sulphate?

I prepare the CHROMagar Col-APSE (for selection of colistin resistant gram-negative bacteria) exactly to manufacturers guidelines. I have E. coli isolates that grow perfectly well on this media,...

26 March 2020 9,377 4 View

DNA gel has a U shape?

22 August 2017 4,812 3 View

Can anyone recommend a proper procedure using mitomyocin C to inactivate mice T-cells in order to perform a proper mixed lymphocyte reaction?

This MLR will be done using two different strains of mice that is why it is necessary to inactivate the T-cells, so that possible proliferation can be observed.

06 October 2016 2,257 3 View

Can anyone help me with my a stent and balloon inflation model in Abaqus?

22 November 2015 6,911 15 View

Can transient transfection using COS-7 cells with a plasmid encoding a neomycin resistance be used a positive transfected cell selection method?

I want to do a transient transfection using COS-7 cells with a plasmid encoding a neomycin resistance, can I use this as a positive transfected cell selection method? COS-7 cells transfect with...

04 March 2015 9,231 5 View

Are cos-7 cells good for patch clamp?

I have been trying to patch transfected cos-7 cells (Kv channel plasmid) and am having an incredibly difficult time getting a seal let alone breaking in. Has anyone every patched with these cells...

10 December 2014 3,117 2 View

How do I optimize PCR reactants?

Hi everyone! Attached you will a EtBR gel showing failed PCR results in lanes 2 and 3, with positive results for a DNA ladder in lane 1 (100 bases). Additionally, I have attached a photo of the...

01 January 1970 3,528 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View