Pre-coated slides are now available from BD Sure Path.It is very good particularly for mono-layered smear preparation  but very costly,each slide costs  Rs.50/-.Egg albumen give you a background staining.Poly L lysine will give you an unevee surface.In my experience APES or charged slides give best result for Immunohistochemistry or immunocytochemistry.

I have not worked with pancreatic tissues personally, but I have worked with humn retinal cells, which are also very delicate. Our experience was that some matrices affected the differentiation of the cells, but where the objective was simply to enhance attachment, without altering the differentiation patterns of the cells, collagen IV, matrigel and laminin gave good results.

I have worked with mice lung tissues, which are very delicate. I think any positively charged slide should work for tissue adherence. I personally use poly L lysine solution for coating of slides.

Still if you feel that it might not be sufficient, then you can double coat your slide with poly L lysine.

Thank you Ifeoma Ezeonu & Nidul Rathod for your valuable answers.

but what could be the fate if i use egg albumin coating?! is it advisable rather than poly-l-lysine? 

I think a disadvantage may be an increased unspecific staining while IHC. How clean is such a product? other proteins mixed? In your position I would first try it with a small batch.

I have worked already with this kind of tissue. I have used silane from Sigma (A3648), you just need to follow the protocol. See the attachment.

I work with diabetic mouse pancreatic tissue. I used a brand of pre-coated slides called Superfrost by Menkel. Although it could also be your processing technique. Are these paraffin embedded or frozen tissue sections?

hi Claretta Sonia D'Souza, i have both paraffin embedded and frozen tissues.

Thank you Caroline, Wathsala, Gudrun for your valuable answers and suggestions.

Yes, Albumin might give you background staining, moreover you wont get a uniform coating in your each batch.

Rather go with poly L lysine solution.

Pre-coated slides are now available from BD Sure Path.It is very good particularly for mono-layered smear preparation  but very costly,each slide costs  Rs.50/-.Egg albumen give you a background staining.Poly L lysine will give you an unevee surface.In my experience APES or charged slides give best result for Immunohistochemistry or immunocytochemistry.

We have had good success with APES. The Poly L lysine coated slides have given us mediocre results. Albumen will give high background staining and thus we avoid this in our protocols.