Caroline Fernandes-Santos

5 Questions 28 Answers 0 Followers

Questions related from Caroline Fernandes-Santos

Can you help me with rat/mouse embryo histology (formaldehyde-paraffin)?

I am working with Wistar rat embryo collected at the 19th day of gestation. I am used to tissue processing, paraffin embedding, and staining, but I would be grateful if someone expert in embryo...

04 April 2019 994 2 View

Can I use a high salt diet to induce cardiac hypertrophy in mice?

There are several diet formulations to induce hypertension in rodents. I would like to know which formulation is the best for inducing left ventricle hypertrophy in C57Bl/6 mice in a short term....

07 July 2014 4,403 3 View

Serum insulin concentration: RIA versus Elisa

I would like to know which is the best method to quantify serum insulin, Radioimmunoassay (RIA) or Elisa?

09 September 2012 8,503 23 View

Protein extraction with TRIzol for Western blot

Is it TRIZol a good method to extract proteins? If so, which would be the best method to quantify proteins after TRIZol extraction? I'm planning to use the TRIzol reagent from ambion Life...

08 August 2012 2,213 9 View

Western blot of endothelial nitric oxide synthase (eNOS) in rat/mice aorta

Does anybody know of a good protocol to extract proteins from rat aorta? I want to see the expression of endothelial proteins such as eNOS. The biggest problem is the amount of tissue I would need...

07 July 2012 9,264 3 View