Nidul Rathod

4 Questions 14 Answers 0 Followers

Questions related from Nidul Rathod

How does using 0.25x TAE buffer instead of 1x TAE at 200V help in better resolution for sepration of DNA fragments in gel electrophoresis?

The expected DNA fragement size is 650bp

04 April 2017 6,208 2 View

Can anyone suggest any quantitative analysis methodology after performing IHC?

I have perform my IHC on cervical cancer tissues (PF), following that I have also got the slides evaluated from the pathologist. The pathologist have scored the slides on the basis of the protein...

03 March 2015 2,927 6 View

Can we perform Immunofluorescence with frozen tissue?

I want to perform colocalization of two markers (stem cell marker & angiogenesis).

11 November 2014 2,898 13 View

How can I stop the tissue getting torn off during an immunohistochemistry procedure?

I am performing IHC on tumor tissue, but during the process, some of the tissue comes off the slide. Slide coating: Tissue bond solution. Antigen retrieval method: Water bath.

07 July 2014 8,947 24 View