As these images shown, black dots seem adherent on my cell ,can be slightly observed under 400× magnification.They cannot be removed by PBS washing or medium repalcement ,and antibiotics ,including penicillin, streptomycin, gentamycin, neomycin , ciproflaxin and purchased myoplasma removing reagents are useless to them. These dots only exist on the cells and could be observed moving under 400× sight ,I am not sure if it is brownian motion. Colleagues in the same lab using same batch of serum or medium never find this phenomenon but we cultured different cells .
I m sure it is not bacteria contamination ,for no colony found on LB plate after 48h of incubation at 37℃ with cultured medium added on. If there 's possible that it is mycoplasma , which is resistant to mycoplasma removing reagents.
Could anybody give me some advice? really appreciate.
you might want to check your cells for mycoplasma. Sometimes they do give a similar phenotype on the cells.
